This study aimed at identifying the effect of using e-Portfolio for developing post graduates' reflective self-assessment, motivation, and achievement. A quasi-experimental pre-post test one group design was employed. An e-portfolio program was developed by the researcher and used with the study group. Participants were thirty males and females. The instruments included a reflective self assessment scale, a motivation scale, and an achievement test. Analysis of data obtained showed that the study group achieved higher in the post administration of the scales, and the achievement test than in the pre-performance. Recommendations and suggestions for further research are presented.