This study was designed to investigate the effect of some factors affecting on in vitro growth enhancement of Topas canola genotype. During establishment stage, MS medium strengths (full and half), explant types (shoot tip and axillary node) and cytokinin types (BA and Kin) were investigated. Using shoot tip explants on full MS medium that supplemented with 1.0 mgl^-1 BA proved to be the best treatment during this stage. In addition, different BA concentrations (0.0, 0.1, 0.5 and 2.5 mgl^-1) and the same previous concentrations of IBA or NAA in the presence of 1.0 mgl^-1 of BA or Kin were examined during multiplication stage. Results indicated that BA proved to be the best cytokinin especially at 2.5 mgl^-1 or at 1.0 mgl^-1 in the presence of 0.5 ^mgl-1 IBA since it produced the maximum number of shoots/explant. During rooting stage, effects of auxin type (IBA and NAA) at different concentrations (0.0, 0.1, 0.5 and 2.5 mgl^-1) and the same previous concentrations of IAA, NAA and 2,4-D in the presence of 0.5 mgl^-1 IBA were evaluated. Generally, using of half MS medium supplemented with 2.5 mgl^-1 IBA individual or 0.5 mgl^-1 IBA in combination with 0.1 mgl^-1 IAA stated to be the most suitable treatments for root induction and growth. Obtained plantlets were successfully acclimatized (70-80% survivability) in peat moss and sand (1:1, v/v) medium in the greenhouse.