ABSTRACT:
The present study aims to integrate the benefits of Illumina
microbial sequencing approaches using novel prepared copper oxide
(CuO) nanoparticles for improving water quality monitoring and
management. Our results showed rapid and accurate discovery of novel
different microbial compositions in the collected water samples. The
domestic drains were had pathogenic microbes as viruses, Alpha-, Beta-
Gamma proteobacteria, Bacteroidia, Deinococci, Bacilli also Clostridia
with variety quantitive. The class Pseudomonadales were found to be
higher in quantity in all drains outfall. Viruses, including Enteroviruses
and hepatitis A and E species were found out in high volume of domestic
drains in comparison with the agricultural and industrial drains. On the
other hand, our results showed that decreasing and removing microbial
pathogens using prepared copper oxide nanoparticles (CuO NPs) by
quick precipitation method. The highest antibacterial activity was notified
for CuO NPs with the optimum concentration 102 μg/mL were ranged
between 92 to 96% after contact time 24h at 35ᵒC. The shaking during the
treatment gave a positive effect removing with CuO NPs. Concluding
remarks highlighted the potential of nanoparticles and Illumina mix as
accurate, simple method. The study recommended future efforts to apply
this as a robust, rapid and powerful metagenomics technology to detecting
other disease-causing agents of public health concern to update and
control water pollution.