ABSTRACT
Probiotics have been widely explored in food processing,
production, research and disease prevention, therefore, the present study
was conducted to evaluate the biological evaluation of probiotic
fermented milk (Rayeb) on obese rats for 8 weeks. The rats (n=35) were
classified into 5 groups, as follows: Group (1): fed on basal diet and kept
as negative control group. While the rats (n= 28) were fed on high fat
diet for four weeks to induce obesity then were divided into four groups,
group (2) was fed on high fat diet only and served as control positive
group. Groups (3 and 4): obese rats were fed on high fat diet and were
given orally 1 ml of fermented milk “Rayeb" and Vitamin D at 600
IU/Kg BW respectively. Group (5): was fed on basal diet and was given
orally a mixture of (1 ml of Rayeb and Vitamin D at 600 IU/Kg BW).
The results indicated that, the supplementation with fermented
milk (Rayeb), vitamin D or their mixture significantly decreased
(P<0.05) the final body weight compared to the positive control group by
26.27%, 21.84%, 30.03%, respectively due to lowering the feed intake
and the feed efficiency ratio of the obese rats. Moreover, the
concentration of leptin hormone as well as the percent of peritoneal fat
bad were significantly lowered (P<0.05) for all treated groups. It was
observed that, the supplementation with the tested food materials
improved the liver and kidney functions as well as lipid profile of the 
obese rats. It could be concluded that, fermented milk (Rayeb), vitamin D
or their mixture might be useful in weight reduction.