ABSTRACT Silybummarianum (SM)(milk thistle, family: Asteraceae)is a therapeuticherb with a 2,000-year history of use. SM seeds extract has been called silymarin which has manytherapeuticactivities asantidiabetic, anti-inflammatory andneuroprotective.The aim of the present study was to investigate the effect of Silybummarianumseeds on hepatotoxicity in rats. Thirty adult male albino rats (Sprague-Dawley strain), weighing about (150±10g)were divided randomly into two main groups as follow: the first group (-ve control= 5 rats) was fed on basal diet. The second group (25 rats) were fed on basal diet and injected with CCl₄ 1ml/kg, (1:1) mixture with paraffin oil for 3 days to induce acute liver damage, then divided into 5 groups from group 2 to group 6. Group 2 (+ve control) fed on basal diet. Group 3 and 4 fed on basal diet supplemented with 5% and 10% SM seeds powder, respectively. Also, group 5 and 6 fed on basal diet supplemented with 5% and 10% SM seeds aqueous extract, respectively. At the end of the experimental period (4 weeks), rats were scarified and serum was collected to determine liver functions, lipid profile and antioxidant enzyme. The results showed that serum levels of alanine aminotransferase (ALT),aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin and malondialdehyide (MDA) were significantly elevated (P