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230343

Microbiological study of some commonly used foods and drinks in Tanta Al Gharbia Governorate

Article

Last updated: 04 Jan 2025

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Subjects

-

Tags

Medical microbiology

Abstract

Background:Food borne illness represents a major public health problem associated with high morbidity and mortality rates. Methods: One hundred samples (foods and drinks) were collected in sterile stomacher bags, Bacterial isolation, colony count, antimicrobial susceptibility testing, and detection of Staphylococcus aureus (S.aureus) enterotoxin genes by multiplex PCR were done. Results: The current study revealed that the Gram-negative bacilli were the most frequent isolated organisms representing (63.3%) followed by Gram positive bacteria (34.5%) and fungi were the least found (2.2%). Among Gram negative bacilli, Klebsiella was the most frequently isolated bacteria, among Gram positive bacteria, Staphylococci were the most frequently isolated bacteria, all isolated fungi were Candida species. Out of 16 identified S. aureus strains recovered from the examined samples, 6 isolates were proved to be enterotoxigenic. One isolate carried Staphylococal enterotoxin C (SEC) gene, two isolates carried both Staphylococcal enterotoxin A (SEA) genes and Staphylococcal enterotoxin B (SEB) gene, and three isolates carried SEA, SEB, and SEC genes. None of the isolates contained SED gene. Conclusion: Based on the findings of this study, food borne illness represent a major public health problem in Tanta.

DOI

10.21608/mid.2022.126007.1255

Keywords

Keywords: contamination of food and drink, Foodborne ilness, Enterotoxins, Multiplex PCR

Authors

First Name

Mai

Last Name

Abd Elkarim

MiddleName

-

Affiliation

Forensic lab doctor in DNA unit in Tanta Department ,the Egyptian Forensic Medicine Authority

Email

maref6662@gmail.com

City

-

Orcid

-

First Name

Eman

Last Name

Hegazy

MiddleName

E.

Affiliation

Medical Microbiology and Immunology Department, Tanta University, Faculty of Medicine, Egypt.

Email

eman.hegazy@med.tanta.edu.eg

City

Tanta

Orcid

-

First Name

kareman

Last Name

Eshra

MiddleName

Ahmed

Affiliation

Medical Microbiology and Immunology Department, Tanta University, Faculty of Medicine, Egypt.

Email

drkaremaneshra2004@hotmail.com

City

Tanta

Orcid

0000-0002-5742-3843

First Name

Afaf

Last Name

Zamzam

MiddleName

Sayed Ahmed

Affiliation

Medical Microbiology and Immunology Department, Tanta University, Faculty of Medicine, Egypt.

Email

afafzamzam@yahoo.com

City

Tanta

Orcid

-

Volume

3

Article Issue

4

Related Issue

37291

Issue Date

2022-11-01

Receive Date

2022-03-07

Publish Date

2022-11-01

Page Start

1,032

Page End

1,043

Print ISSN

2682-4132

Online ISSN

2682-4140

Article File

MID_Volume 3_Issue 4_Pages 1032-1043.pdf

PDF . 414.6kB

Link

https://mid.journals.ekb.eg/article_230343.html

Detail API

https://mid.journals.ekb.eg/service?article_code=230343

Order

30

Type

Original Article

Type Code

1,157

Publication Type

Journal

Publication Title

Microbes and Infectious Diseases

Publication Link

https://mid.journals.ekb.eg/

MainTitle

Microbiological study of some commonly used foods and drinks in Tanta Al Gharbia Governorate

Details

Type

Article

Created At

22 Jan 2023