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145562

Optimization of Aeration and Agitation for α-amylase production by Aspergillus flavus from water hyacinth

Article

Last updated: 25 Dec 2024

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Abstract

Amylases are a group of commercially important enzymes with various clinical, medical and biotechnological applications. The current study aims to optimize the agitation and aeration (oxygen transfer) conditions for α-amylase production by Aspergillus flavus using water hyacinth extract; as an available waste and very cheap nutritional substance, in a lab scale stirred tank bioreactor (submerged fermentation). Data showed that the maximal cell biomass and enzyme specific activity were reached at low aeration rate (0.5 v/v/m) and moderate agitation speed (200 rpm). The maximum specific amylase production rate has been reached after 24 hrs. In addition, it has been confirmed that scaling-up Aspergillus flavus does not, inversely, affect α-amylase productivity compared with flask level production. Aspergillus flavus has been approved as promising fungal isolate for production of a-amylase using water hyacinth for application in many fields.    

DOI

10.21608/ijisd.2021.145562

Keywords

α-amylase, fermentation, Bioreactor, Aeration, agitation, Water hyacinth

Authors

First Name

Enas

Last Name

Mostafa

MiddleName

-

Affiliation

chemical Engineering and Pilot Plant Department, National Research Centre, Egypt

Email

drenasmostafa@gmail.com

City

-

Orcid

-

First Name

Mostafa

Last Name

Abo Elsoud

MiddleName

-

Affiliation

National Research Centre

Email

masnrc@gmail.com

City

Giza

Orcid

0000-0002-4192-9495

First Name

Marwa

Last Name

Salem

MiddleName

S.

Affiliation

department of Botany and Microbiology, Faculty of Science, Al Azhar University (Girls Branch), Cairo, Egypt

Email

mereamsalah@gmail.com

City

-

Orcid

-

First Name

Eman

Last Name

El-Tabakh

MiddleName

-

Affiliation

Department of Botany and Microbiology, Faculty of Science, Al Azhar University (Girls Branch), Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Nagwa

Last Name

Sidkey

MiddleName

M.

Affiliation

Department of Botany and Microbiology, Faculty of Science, Al Azhar University (Girls Branch), Cairo, Egypt

Email

nagwa.sidkey@nagwasedkey.com

City

-

Orcid

-

Volume

2

Article Issue

1

Related Issue

21476

Issue Date

2021-07-01

Receive Date

2020-09-01

Publish Date

2021-07-01

Page Start

25

Page End

35

Print ISSN

2682-3993

Online ISSN

2682-4000

Article File

IJISD_Volume 2_Issue 1_Pages 25-35.pdf

PDF . 549.1kB

Link

https://ijisd.journals.ekb.eg/article_145562.html

Detail API

https://ijisd.journals.ekb.eg/service?article_code=145562

Order

3

Type

Original Article

Type Code

1,141

Publication Type

Journal

Publication Title

International Journal of Industry and Sustainable Development

Publication Link

https://ijisd.journals.ekb.eg/

MainTitle

Optimization of Aeration and Agitation for α-amylase production by Aspergillus flavus from water hyacinth

Details

Type

Article

Created At

22 Jan 2023