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211355

Microbiological and genetical identification of some vermicompost beneficial associated bacteria

Article

Last updated: 04 Jan 2025

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Subjects

-

Tags

Botany and genetic sciences

Abstract

n the present work, isolation and identification of some plant beneficial bacteria of vermicompost were conducted. A total of ten bacterial isolates were selected and characterized morphologically and biochemically. Molecular characterization of the bacterial isolates using 16S rRNA gene sequence analysis and phylogenic tree were conducted. According to the percentages of homology of 16S rRNA sequence with the closest NCBI strain, all of the ten bacterial isolates were belonged to six species (B. subtilis, B. atrophaeus, B. hemicentroti, B. velezensis, B. nakamurai and B. amyloliquefaciens) of genus Bacillus. Except isolate YFV05 which showed only 99.77% of homology with Bacillus subtilis strain NRRL NRS-744, all of the other isolates showed 100% homology to different strains of Bacillus spp < /em>. Generally, results revealed that all the ten bacterial isolates had different levels of in vitro antagonistic effect against four phytopathogenic fungi (Fusarium solani, Fusarium spp, Macrophomina phasolenia, and Rhizoctonia solani). In addition, treatment with YFV014, YFV05 and YFV012 isolates offered the highest values of vigor index (2166.0, 1775.0 and 1733.6), respectively. Finally, it could be concluded that plant beneficial bacteria might be isolate from vermicompost which in turn can be used to improve plant growth directly by production of plant growth-regulating hormones and enzymes and indirectly by controlling plant pathogens and other pests, thereby enhancing plant health and minimizing the yield loss of various crops.

DOI

10.21608/svuijas.2021.106875.1154

Keywords

16S rRNA, Bacillus spp, gene sequencing, vermicompost

Authors

First Name

H.A.H.

Last Name

Soltan

MiddleName

-

Affiliation

Central Laboratory of Organic Agriculture, Agriculture Research Center, 12619 Giza, Egypt

Email

soloo2525@gmail.com

City

Giza

Orcid

0000-0002-3079-8522

First Name

O.F.

Last Name

Dakhly

MiddleName

-

Affiliation

Genetics Department, Faculty of Agriculture, Minia University, 61519 Minia, Egypt

Email

omar_dakhly@mu.edu.eg

City

Minia

Orcid

-

First Name

M.A.

Last Name

Mahmoud

MiddleName

-

Affiliation

Genetics Department, Faculty of Agriculture, Minia University, 61519 Minia, Egypt

Email

mahmoud_mah@yahoo.com

City

Minia

Orcid

-

First Name

Ysamen F.M.

Last Name

Fayz

MiddleName

-

Affiliation

Genetics Department, Faculty of Agriculture, Minia University, 61519 Minia, Egypt

Email

yasmyn.fayz@mu.edu.eg

City

Minia

Orcid

-

Volume

4

Article Issue

1

Related Issue

28927

Issue Date

2022-01-01

Receive Date

2021-12-03

Publish Date

2022-01-01

Page Start

21

Page End

36

Print ISSN

2636-3801

Online ISSN

2636-381X

Article File

SVUIJAS_Volume 4_Issue 1_Pages 21-36.pdf

PDF . 909.2kB

Link

https://svuijas.journals.ekb.eg/article_211355.html

Detail API

https://svuijas.journals.ekb.eg/service?article_code=211355

Order

3

Type

Original Article

Type Code

1,132

Publication Type

Journal

Publication Title

SVU-International Journal of Agricultural Sciences

Publication Link

https://svuijas.journals.ekb.eg/

MainTitle

Microbiological and genetical identification of some vermicompost beneficial associated bacteria

Details

Type

Article

Created At

22 Jan 2023