The aim of this RCT is to study the effect of low level laser therapy (LLLT) with en masse maxillary anterior retraction in class II division 1 female patients on root resorption. Materials and Methods: 20 non-growing female patients were randomly allocated in 2 groups: Group I receiving LLLT 4 times/month (0, 3, 7 and, 14) days of activation, Group II receiving no laser therapy. CBCT was taken at T1 (prior to retraction) and T2 (after closure of spaces). Root resorption was evaluated by analyzing both root length (RL) and root area (RA) of six anterior teeth and compared between T1 and T2. Results: Root resorption was detected in all teeth in both groups with significant difference between the two groups regarding RL and RA (P<0.01).No significant difference recorded between the percentage change in RL and RA in both groups. Conclusion: Low-level laser therapy had no effect whether directly or indirectly on amount of apical root resorption during in masse retraction.