The current study examined the importance of teaching FL culture in the Egyptian FL context. It investigated whether gender is statistically a significant contributor to the Egyptian EFL pre-service teachers' perception of FL culture instruction. It discussed how the Egyptian EFL male and female pre-service teachers might vary in their perception of teaching FL culture to the Egyptian EFL learners. As for the participants, one hundred Egyptian EFL pre-service teachers participated in the present study. They were enrolled at the faculty of Education at two Egyptian universities: Matrouh University and October 6 University. They were divided into two groups with 50 participants each based on gender. Data were collected through a 20-item questionnaire that was divided into four sections. Data were analyzed according to the descriptive statistics and one way analysis of variance (ANOVA). Findings indicated that gender significantly contributed to the perception of Egyptian EFL pre-service of FL culture instruction.