The purpose of this study is to evaluate water soluble vitamins (C, B₁, B₂, B₃, B₆, B₉ and B₁₂) in honey from honey bees feed on Spring flowers, Sweet marjoram, Sun flower, Clover flowers, Citrus fruits and Black seed using gradient RP-HPLC methods with PDA detector. The highest levels of vitamins C, B₁, B₂, and B₆ were observed in citrus fruits honey, sunflower honey, black seed honey and spring flower honey, respectively. The highest levels in vitamins B₃, B₉ and B₁₂ were observed in sweet marjoram honey, where the lowest levels for these vitamins were observed in clover flower honey. The lowest levels for vitamins B₂ and B₉ were observed in black seed honey. The lowest levels for vitamins C and B₁ were observed in spring flower honey and citrus fruits, respectively. These results indicated the presence of a significant difference in water soluble vitamins content depending on the type of Egyptian honey.