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239242

EFFECT OF UV-C STRESS ON TOTAL PHENOLICS, TOTAL FLAVONOIDS AND KHELLIN CONTENTS IN AMMI VISNAGA (L.) LAM. FRUITS

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Last updated: 25 Dec 2024

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Abstract

Ammi visnaga fruits (L.) Lam., is widely used for its antispasmodic and vasodilating effects. These effects are attributed to its main content of khellin. In our research, we studied the effect of UV-C stress on the metabolite production of the fruits. Fruits were subjected to UV-C treatment for ½, 1, 2, 3, 4 and 5 hr The change in the metabolic profile was studied by the quantification of total phenolics, total flavonoids and khellin in the methanolic extracts of the fruits applied to stress and extracts of control fruits. Total phenolics and total flavonoids were quantified colorimetrically using appropriate reagent for each. Folin-Ciocalteau reagent was applied for the quantification of total phenolics while 2% AlCl3 solution was applied for the quantification of flavonoids.  On the other hand, khellin was quantified by HPLC analysis. Our results showed that the concentrations of total phenolics, total flavonoids and khellin contents of the fruit extracts are significantly increased by exposing the fruits to UV-C stress with maximum production at 1 and 2 hr.

DOI

10.21608/bfsa.2022.239242

Keywords

Ammi visnaga, Folin-Ciocalteau reagent, Khellin, UV-C stress, Total Phenolics, Total flavonoids

Authors

First Name

Iman

Last Name

Khallaf

MiddleName

S. A.

Affiliation

Pharmacognosy Department, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt

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Orcid

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First Name

Rania

Last Name

Hussein

MiddleName

A. A.

Affiliation

Student at Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt

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Orcid

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First Name

Osama

Last Name

Ali

MiddleName

M. M.

Affiliation

Student at Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt

Email

oosama.mohamed.assiut@gmail.com

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-

Orcid

-

First Name

Gehad

Last Name

Remeih

MiddleName

S.

Affiliation

Student at Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt

Email

gs8501555@gmail.com

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-

Orcid

-

First Name

Aya

Last Name

Mortada

MiddleName

S.

Affiliation

Student at Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt

Email

aya.sayed.mortada@gmail.com

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-

Orcid

-

First Name

Khaled

Last Name

Mohamed

MiddleName

M.

Affiliation

Pharmacognosy Department, Faculty of Pharmacy, Fayoum University, Fayoum, 63514, Egypt

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-

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Volume

45

Article Issue

1

Related Issue

34403

Issue Date

2022-06-01

Receive Date

2021-09-14

Publish Date

2022-06-01

Page Start

89

Page End

97

Print ISSN

1110-0052

Online ISSN

3009-7703

Article File

BFSA_Volume 45_Issue 1_Pages 89-97.pdf

PDF . 645.4kB

Link

https://bpsa.journals.ekb.eg/article_239242.html

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https://bpsa.journals.ekb.eg/service?article_code=239242

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Type

Original Article

Type Code

1,096

Publication Type

Journal

Publication Title

Bulletin of Pharmaceutical Sciences Assiut University

Publication Link

https://bpsa.journals.ekb.eg/

MainTitle

EFFECT OF UV-C STRESS ON TOTAL PHENOLICS, TOTAL FLAVONOIDS AND KHELLIN CONTENTS IN AMMI VISNAGA (L.) LAM. FRUITS

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Article

Created At

22 Jan 2023