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174146

OPTIMIZATION OF CULTURE CONDITIONS FOR KOJIC ACID PRODUCTION IN SURFACE FERMENTATION BY ASPERGILLUS ORYZAE ISOLATED FROM WHEAT GRAINS

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Last updated: 25 Dec 2024

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Abstract

Kojic acid is a natural organic acid synthesized during aerobic fermentation of carbohydrates as a secondary metabolite by some species of Aspergillus and has been used commercially in several industrial applications. The current investigation aims to optimize the culture conditions for kojic acid production from starch as a carbon source by the novel isolate Aspergillus oryzae 1034. Seventy-five isolates representing 11 species of Aspergillus were isolated from stored wheat grains and screened for kojic acid biosynthesis. Amongst, A. oryzae 1034 was selected as the most potent kojic acid producer from starch. This strain was subjected to different fermentation conditions to maximize the kojic acid production from starch versus glucose. The results concluded that glucose and starch substrates in concentrations 60 and 80 g/l, respectively were the optima for kojic acid production. The optimum phosphorus concentration was 0.5 and 2.0 g/l KH2PO4 in glucose and starch media, respectively. The maximum kojic acid yield was attained at 28ºC for 11 days of incubation in both glucose and starch media with pH 4.5 and 5.0, respectively. Supplementation of Pb+2 to glucose medium and Zn+2 to starch medium stimulated the biosynthesis of kojic acid to 79.3 and 68.8 g/l, respectively. Alternatively, kojic acid biosynthesis was decreased by supplementation of amino acids in the fermentation medium. These findings suggest the possibility of using A. oryzae 1034 as a promising.

DOI

10.21608/bfsa.2021.174146

Authors

First Name

Abdel-Hamied

Last Name

Rasmey

MiddleName

M.

Affiliation

Department of Botany & Microbiology, Faculty of Science, Suez University, Suez 43518, Egypt

Email

am_rasmey@yahoo.com

City

-

Orcid

-

First Name

Marwa

Last Name

Abdel-Kareem

MiddleName

-

Affiliation

Department of Botany & Microbiology, Faculty of Science, Sohag University, Sohag 82524, Egypt

Email

marwaabdelkareem7@gmail.com

City

-

Orcid

-

Volume

44

Article Issue

1

Related Issue

25283

Issue Date

2021-06-01

Receive Date

2021-02-02

Publish Date

2021-06-01

Page Start

201

Page End

211

Print ISSN

1110-0052

Online ISSN

3009-7703

Article File

BFSA_Volume 44_Issue 1_Pages 201-211.pdf

PDF . 483.3kB

Link

https://bpsa.journals.ekb.eg/article_174146.html

Detail API

https://bpsa.journals.ekb.eg/service?article_code=174146

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20

Type

Original Article

Type Code

1,096

Publication Type

Journal

Publication Title

Bulletin of Pharmaceutical Sciences Assiut University

Publication Link

https://bpsa.journals.ekb.eg/

MainTitle

OPTIMIZATION OF CULTURE CONDITIONS FOR KOJIC ACID PRODUCTION IN SURFACE FERMENTATION BY ASPERGILLUS ORYZAE ISOLATED FROM WHEAT GRAINS

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Article

Created At

22 Jan 2023