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69698

MICROBIAL TRANSFORMATION OF LIPIDS: SELECTIVE HYDRATION OF OLEIC ACID

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Last updated: 25 Dec 2024

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Abstract

The cultures Nocardia sp. NRRL 5646, Nocardia aurantia ATCC 12674, and Mycobacterium fortium AM 53378 were identified as being capable of metabolizing oleic acid. These organisms selectively hydrated the cis-double bond of oleic acid in the presence of other fatty acids like oleic : linoleic (1 :1, w/w), oleic : stearic (9:1, w/w), oleic : linoleic : stearic (2:2:1,w/w), and oleic:linoleic: palmitic (2:2:1, w/w). Based on gas chromatography of the fatty acid methyl esters, it was found that stearic, palmitic, and linoleic acids were unreactive with the hydratase enzyme of the three microbes and in all cases 80-88% of oleic acid was consumed. Thus, the hydratase enzyme was able to selectively and quantitatively hydrate oleic acid and discriminate among monoenoic, dienoic and saturated fatty acids. This work provides a mean of oxidatively removing oleic acid from among a mixture of other mono and polyunsaturated vegetable oil fatty acids. We believe that the selected organisms have the abilities to transform oleic acid and to selectively utilize that compound from similar mixtures as well.

DOI

10.21608/bfsa.1995.69698

Authors

First Name

Saleh

Last Name

El-Sharkawy

MiddleName

H.

Affiliation

Department of Pharmacognosy, Faculty of Pharmacy, El-Mansoura University, El-Mansoura, El-Mansoura, Egypt

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Orcid

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First Name

John

Last Name

Rosazza

MiddleName

P. N.

Affiliation

Center for Biocatalysis, College of Pharmacy, Iowa University, Iowa City, IA 52242 USA

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Volume

18

Article Issue

2

Related Issue

10588

Issue Date

1995-12-01

Receive Date

1995-11-07

Publish Date

1995-12-31

Page Start

103

Page End

106

Print ISSN

1110-0052

Online ISSN

3009-7703

Article File

BFSA_Volume 18_Issue 2_Pages 103-106.pdf

PDF . 262.0kB

Link

https://bpsa.journals.ekb.eg/article_69698.html

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https://bpsa.journals.ekb.eg/service?article_code=69698

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Original Article

Type Code

1,096

Publication Type

Journal

Publication Title

Bulletin of Pharmaceutical Sciences Assiut University

Publication Link

https://bpsa.journals.ekb.eg/

MainTitle

MICROBIAL TRANSFORMATION OF LIPIDS: SELECTIVE HYDRATION OF OLEIC ACID

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Article

Created At

22 Jan 2023