E. coli transmitted from under-cooked hamburger may cause life threatening conditiosn including hemorrhagic colitis, hemolytic uremic syndrome and thrombotic-thrombocytopaenic purpurea. Unfortunately, the emergence of resistance has become increased. Therefore, the antimicrobial susceptibility of the causative strain E. coli "O157:H7" was determined in this study. Results showed that E. coli O157:H7 was resistant to all tested antibiotics except danofloxacin. Therefore, this study recommend the use of danofloxacin in controlling infections caused by E. coli transmitted from hamburger.