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110205

MOLECULAR CHARACTERIZATION OF SOME GRAM-NEGATIVE BACTERIA ISOLATED FROM MILK

Article

Last updated: 25 Dec 2024

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Subjects

-

Tags

Animal Hygiene, Husbandry, Production, Genetics, Economics, Nutrition and Food Control.

Abstract

Milk isn't only a perfect food, but also an ideal media for microbes, thus it is a major pathogen vehicle. This study was conducted to characterize molecularly some of Gram-negative pathogens in random150 raw milk samples (100 from healthy dairy farm animals and 50 from milk vendors) collected from ElmahallaElkobra and Mansoura cities, Egypt, then 20 farm samples with subclinical mastitis were excluded. Gram-negative bacteria were isolated and identified biochemically from the other 130 samples; the predominant species percentages were Escherichia coli 25.4, Serratiamarcescens 13.8, Kluyveraascorbata 8.5, Citrobacterdiversus 7.7, Klebsiellaoxytoca 5.4, and Klebsiellapneumoniae3.8. 18 shigatoxin producingE. coli (STEC) strains were classified serologically to 8 non-O157 serotypes, and the predominant was O26:H11 (27.78%). By PCR;16S-23S rDNA was identified in 3 K. pneumoniae, and shiga toxins (stx1 and 2), α-haemolysin (hlyA) and intimin (eaeA) genes were detected in 15, 13, 7 and 9 STEC respectively. Conclusively, raw milk still contributes a public hazard,so, better effective control measures are required.

DOI

10.21608/kvmj.2018.110205

Keywords

Molecular characterization, Gram-negative pathogens, Milk

Authors

First Name

Huda H. A.

Last Name

Borham

MiddleName

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Affiliation

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Email

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City

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Orcid

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First Name

Salwa M.

Last Name

Helmy

MiddleName

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Affiliation

Department of Bacteriology, mycology and immunology, Faculty of Veterinary Medicine, Kafrelsheikh University

Email

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City

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Orcid

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First Name

Ibrahim

Last Name

El-hawary

MiddleName

-

Affiliation

Faculty of Aquatic Wealth and Fisheries, Kafrelsheikh University

Email

-

City

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Orcid

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Volume

16

Article Issue

1

Related Issue

16723

Issue Date

2018-04-01

Receive Date

2018-08-01

Publish Date

2018-04-01

Page Start

55

Page End

69

Print ISSN

1687-1456

Online ISSN

2682-2954

Article File

KVMJ_Volume 16_Issue 1_Pages 55-69.pdf

PDF . 709.6kB

Link

https://kvmj.journals.ekb.eg/article_110205.html

Detail API

https://kvmj.journals.ekb.eg/service?article_code=110205

Order

4

Type

Original Article

Type Code

1,064

Publication Type

Journal

Publication Title

Kafrelsheikh Veterinary Medical Journal

Publication Link

https://kvmj.journals.ekb.eg/

MainTitle

MOLECULAR CHARACTERIZATION OF SOME GRAM-NEGATIVE BACTERIA ISOLATED FROM MILK

Details

Type

Article

Created At

22 Jan 2023