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110206

MOLECULAR DETECTION OF STAPHYLOCOCCUS AUREUS ENTEROTOXINS ISOLATED FROM MASTITIC SHE-CAMELS

Article

Last updated: 04 Jan 2025

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Subjects

-

Tags

Veterinary Microbiology and Parasitology.

Abstract

A total of  150 raw milk samples were collected from she camels from Aswan, 100 from individual breed in Daraw and 50 from pastoral camels in Shalateen in Egypt. First, clinical examination revealed no cases with clinical mastitis. Second, the collected milk samples were tested by California Mastitis Test and the results revealed that the percent of subclinical mastitis was (39.3%) for total number of milk samples, but the ratio in Daraw (48%) higher than  in Shalateen (22%). Third, by conventional culture method, 20 % of samples were positive to Staphylococcus spp. Fourth, Staphylococcal isolates were identified by conventional biochemical test, (46.7%)  of these isolate showed positive for coagulase test. Fifth, these coagulase positive isolates submitted for molecular identification targeting 16S rRNA gene (Staphylococcus genus specific), nuc gene (S. aureus species specific) and  Staphylococcal enterotoxins genes (SEA, SEB andSEC) by multiplex PCR, (92.9%) positive for 16s rRNA and confirmed as Staphylococcus spp., (64.3%) positive for nuc gene and confirmed to be S.aureus and (50%) Staphylococcus isolates were enterotoxin-positive to sea, (35.7%) of them for S.aureus strains  and  (14.3%)  for other coagulase positive Staphylococcus spp. Finally, these isolates were submitted for antibiotic sensitivity testing by using various antibiotics and the proportion of isolates resistance to the antibiotics were penicillin G and chloramphenicol (100%), colistin (93%), tetracycline and lincomycin (78.6%), oxalinic acid (71.4%), amoxicillin (50%), neomycin and cefaclor (42.8%), erythromycin (35.7%), ciprofloxacin (28.6%) and gentamycin (21.4%).

DOI

10.21608/kvmj.2018.110206

Keywords

Staphylococcus spp, Mastitic She-Camels

Authors

First Name

Mai A.S.

Last Name

Boghdady

MiddleName

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Affiliation

Animal Health Researches Institute, Aswan branch, Egypt

Email

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City

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Orcid

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First Name

Alsagher .O.A.

Last Name

Ali

MiddleName

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Affiliation

Department of Animal Medicine, faculty of Veterinary Medicine, South Valley University, Qena city, Qena 83523, Egypt.

Email

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City

-

Orcid

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First Name

Hassan. Y.A. H.

Last Name

Mahmoud

MiddleName

-

Affiliation

Department of Animal Medicine, faculty of Veterinary Medicine, South Valley University, Qena city, Qena 83523, Egypt.

Email

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City

-

Orcid

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First Name

Khaled I.

Last Name

El-Ekhnawy

MiddleName

-

Affiliation

Animal Health Researches Institute, Dokki, Giza, Egypt

Email

-

City

-

Orcid

-

First Name

Adel E.A.

Last Name

Mohamed

MiddleName

-

Affiliation

Department of Animal Medicine, faculty of Veterinary Medicine, South Valley University, Qena city, Qena 83523, Egypt.

Email

-

City

-

Orcid

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Volume

16

Article Issue

2

Related Issue

16720

Issue Date

2018-10-01

Receive Date

2018-07-01

Publish Date

2018-10-01

Page Start

23

Page End

43

Print ISSN

1687-1456

Online ISSN

2682-2954

Article File

KVMJ_Volume 16_Issue 2_Pages 23-43.pdf

PDF . 666.4kB

Link

https://kvmj.journals.ekb.eg/article_110206.html

Detail API

https://kvmj.journals.ekb.eg/service?article_code=110206

Order

2

Type

Original Article

Type Code

1,064

Publication Type

Journal

Publication Title

Kafrelsheikh Veterinary Medical Journal

Publication Link

https://kvmj.journals.ekb.eg/

MainTitle

MOLECULAR DETECTION OF STAPHYLOCOCCUS AUREUS ENTEROTOXINS ISOLATED FROM MASTITIC SHE-CAMELS

Details

Type

Article

Created At

22 Jan 2023