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110142

MOLECULAR CHARACTERIZATION OF ANTIBIOTIC RESISTANCE IN GRAM – NEGATIVE BACTERIA ISOLATED FROM DAIRY PRODUCTS

Article

Last updated: 25 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Animal Hygiene, Husbandry, Production, Genetics, Economics, Nutrition and Food Control.

Abstract

The present work was carried out on 127 dairy products samples (62 buffalo milk, 46 cow milk, 16 kariesh cheeses and 3 yoghurt) were obtained from retail markets in Dakahlia governorate. (214) isolates were assayed for antimicrobial susceptibility, the presence of integrons and antimicrobial resistance genes. 152 out of 214 (71.02%) Gram – negative bacterial isolates showed multidrug resistance phenotypes for two or more of the following antimicrobial agents: ampicillin, streptomycin, gentamicin, tetracycline, trimethoprim/ sulfamethoxazole, nalidixic acid, ciprofloxacin, amoxicillin - clavulanic acid , chloramphenicol and cefotaxime . PCR screening for integrons showed that  eight (3.73%) isolates of (Enterobacter aerogens, Klebsiella pneumoniae, Klebsiella oxytoca, Citrobacter diversus, Citrobacter freundii, Proteus vulgaris, Escherichia coli and Serratia marsescens) were positive for class 1 integron and all isolates were negative for class 2 integron .β-lactamase resistance gene blaTEM was identified in 6 (2.80%) isolates of (K. oxytoca , E. coli , S. liquefaciens ,E. cloacae, K. pneumoniae and C. diversus). blaCTX-M  was  identified in 3 (1.40%) isolates of
(E. coli , K. oxytoca, and S. marsescens).All isolates were negative for blaCMY.
These results highlighted the role of antimicrobial use in dairy animals and development of transferable gene in bacteria in dairy animals from which such genes can be disseminated by horizontal gene transfer to other bacteria and reach human pathogens.

DOI

10.21608/kvmj.2014.110142

Keywords

Integrons, β-lactamases, blaTEM. blaCTX-M, blaCMY

Authors

First Name

Amany

Last Name

M.I.

MiddleName

-

Affiliation

Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Kafrelsheikh University

Email

-

City

-

Orcid

-

First Name

Hisham

Last Name

S.N.

MiddleName

-

Affiliation

Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Kafrelsheikh University

Email

-

City

-

Orcid

-

First Name

Ahmed

Last Name

A.E.

MiddleName

-

Affiliation

Animal Health Research Institute, Mansoura Laboratory

Email

-

City

-

Orcid

-

Volume

12

Article Issue

2

Related Issue

16455

Issue Date

2014-10-01

Receive Date

2014-07-01

Publish Date

2014-10-01

Page Start

39

Page End

61

Print ISSN

1687-1456

Online ISSN

2682-2954

Article File

KVMJ_Volume 12_Issue 2_Pages 39-61.pdf

PDF . 759.3kB

Link

https://kvmj.journals.ekb.eg/article_110142.html

Detail API

https://kvmj.journals.ekb.eg/service?article_code=110142

Order

3

Type

Original Article

Type Code

1,064

Publication Type

Journal

Publication Title

Kafrelsheikh Veterinary Medical Journal

Publication Link

https://kvmj.journals.ekb.eg/

MainTitle

MOLECULAR CHARACTERIZATION OF ANTIBIOTIC RESISTANCE IN GRAM – NEGATIVE BACTERIA ISOLATED FROM DAIRY PRODUCTS

Details

Type

Article

Created At

22 Jan 2023