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108855

MOLECULAR CHARACTERIZATION OF NEWCASTLE DISEASE VIRUS ISOLATED FROM SAUDI ARABIA

Article

Last updated: 04 Jan 2025

Subjects

-

Tags

Veterinary Medicine and Infectious Diseases “Veterinary Diseases".

Abstract

A total of 34 samples were collected from chicken and pigeon flocks suspected to have Newcastle disease. NDV virus was isolated and identified by inoculation into the allantoic cavity of Specific–pathogen- free chicken eggs followed by hemagglutination and hemagglutination inhibition test. Furthermore, these isolates were geno-typically analysed using RT-PCR with specific primers flanking a  270 bp region representing the hypervariable region of F gene and the PCR-product was sequenced and compared to the previously published sequences. In this study, the F protein of 34 Saudi NDV isolates phylogram revealed clustering with NDVs representing lineage 2,4 and 5.

DOI

10.21608/kvmj.2016.108855

Keywords

Newcastle Disease virus, chicken and pigeon flocks

Authors

First Name

Mahmoud M.

Last Name

Ismail

MiddleName

-

Affiliation

Dept. of Poultry Diseases, college of Veterinary Medicine, Kafrelsheikh University, Egypt. Dept. of Clinical Studies, College of Veterinary Medicine and Animal Resources, King Faisal University, Al-Hasa, Saudi Arabia

Email

-

City

-

Orcid

-

First Name

Abdulrahman

Last Name

Alankari

MiddleName

-

Affiliation

Dept. of Clinical Studies, College of Veterinary Medicine and Animal Resources, King Faisal University, Al-Hasa, Saudi Arabia

Email

-

City

-

Orcid

-

First Name

Mahmoud H. A.

Last Name

Mohamed

MiddleName

-

Affiliation

Dept. of Clinical Studies, College of Veterinary Medicine and Animal Resources, King Faisal University, Al-Hasa, Saudi Arabia

Email

-

City

-

Orcid

-

First Name

Faris

Last Name

Elkhayat

MiddleName

-

Affiliation

Dept. of Poultry Diseases, college of Veterinary Medicine, Kafrelsheikh University, Egypt

Email

-

City

-

Orcid

-

Volume

14

Article Issue

1

Related Issue

16421

Issue Date

2016-05-01

Receive Date

2016-08-20

Publish Date

2016-05-01

Page Start

257

Page End

267

Print ISSN

1687-1456

Online ISSN

2682-2954

Link

https://kvmj.journals.ekb.eg/article_108855.html

Detail API

https://kvmj.journals.ekb.eg/service?article_code=108855

Order

15

Type

Original Article

Type Code

1,064

Publication Type

Journal

Publication Title

Kafrelsheikh Veterinary Medical Journal

Publication Link

https://kvmj.journals.ekb.eg/

MainTitle

MOLECULAR CHARACTERIZATION OF NEWCASTLE DISEASE VIRUS ISOLATED FROM SAUDI ARABIA

Details

Type

Article

Created At

22 Jan 2023