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108683

PHENOTYPIC AND GENOTYPING CHARACTERIZATION OF PASTEURELLA MULTOCIDA IN FARM ANIMALS

Article

Last updated: 25 Dec 2024

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Subjects

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Tags

Veterinary Medicine and Infectious Diseases “Veterinary Diseases".

Abstract

Pasteurellamultocida  which  is  the  common  cause  of  respiratory  disease  in  cattle  causing  higheconomic losses. To achieve this objective,  this study succeeded in isolating  a total of27 of P. multocida isolates from 280 samples (9.6%). Lung tissues showed the highestpercentage  of  P.  multocida  isolation  (13.1%),  followed  by  Nasal Swabs  (5%). These isolates were confirmed  microscopically  to  be  P. multocida  bystaining with Gram's  and  Leishman's  stains,  then  biochemically by traditional test likeindole,  catalase,  oxidase,  urease,  citrate  utilization,  sugar  fermentation  and  gelatinliquefaction tests. Also, the results were confirmed using commercial test API 20E andPCR.  For  common gene and capsular serotyping  of  P. multocida  by using multiplex capsular PCR typing system,  a  total  of 9 representative isolates  weretested and 5  isolates were positive for common gene of P. multocida and positive for capsular type E. Positive P. multocida isolates were tested for presence of virulence factors by multiplex PCR for virulence genotyping which represented by virulence genes (TbpA, nanH, ompH and ptfA) which revealed 100% percentages of Tbpa and nanH in lung tissues and nasal swabs in cattle and buffaloes isolates, the presence of ptfA gene in high percentages in lung tissues in buffalo more than in nasal swabs.

DOI

10.21608/kvmj.2016.108683

Keywords

Pasteurella multocida, Phenotypic, Genotyping, farm animals

Authors

First Name

Tamer I.

Last Name

Bostan

MiddleName

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Affiliation

Animal health research institute, Tanta, Egypt.

Email

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City

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Orcid

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First Name

Helmy A.

Last Name

Torky

MiddleName

-

Affiliation

Department of Bacteriology, Mycology & Immunology, Faculty of Veterinary Medicine, Alexandria University.

Email

-

City

-

Orcid

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First Name

Ashraf M.

Last Name

Ahmed

MiddleName

-

Affiliation

Department of Bacteriology, Mycology & Immunology, Faculty of Veterinary Medicine, Kafrelsheikh University.

Email

-

City

-

Orcid

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First Name

Omnia F.

Last Name

Hassan

MiddleName

-

Affiliation

Bacteriology Research Department, Animal Health Research Institute, Dokki, Egypt

Email

-

City

-

Orcid

-

Volume

14

Article Issue

1

Related Issue

16421

Issue Date

2016-05-01

Receive Date

2016-02-01

Publish Date

2016-05-01

Page Start

99

Page End

113

Print ISSN

1687-1456

Online ISSN

2682-2954

Article File

KVMJ_Volume 14_Issue 1_Pages 99-113.pdf

PDF . 763.6kB

Link

https://kvmj.journals.ekb.eg/article_108683.html

Detail API

https://kvmj.journals.ekb.eg/service?article_code=108683

Order

6

Type

Original Article

Type Code

1,064

Publication Type

Journal

Publication Title

Kafrelsheikh Veterinary Medical Journal

Publication Link

https://kvmj.journals.ekb.eg/

MainTitle

PHENOTYPIC AND GENOTYPING CHARACTERIZATION OF PASTEURELLA MULTOCIDA IN FARM ANIMALS

Details

Type

Article

Created At

22 Jan 2023