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108554

DETECTION OF STAPHYLOCOCCUS AUREUS CLASSIC ENTEROTOXIN GENES IN SOME MEAT PRODUCTS USING MULTIPLEX PCR

Article

Last updated: 25 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Veterinary Microbiology and Parasitology.

Abstract

A total of 200 meat product samples of beef burger, sausage, minced meat and luncheon were randomly collected from different shops and markets in El-Gharbia and Kafr-El-Sheikh governorates. The samples from 3 brands A, B and C were examined for the presence S. aureus using classical bacteriological and molecular methods. The obtained results revealed thatthe 2, 1 and 4 samples of beef burger, sausage and minced meat respectively, were positive for S. aureus by PCR. Additionally multiplex PCR was used for detection of five S. aureus virulence classic enterotoxin genes (sea, seb, sec, sed and see). The obtained results revealed thatthe beef burger samples harbored (1) seb gene; meanwhile sausage sample harbored (1) sea gene and minced meat samples harbored (2) sea, (1) sec and (1) sed genes. This study offers the basis for further phenotypic and molecular characterization S. aureus isolated from these products to guarantee safe consumption of meat products.

DOI

10.21608/kvmj.2016.108554

Keywords

S. aureus, meat products, virulence genes and PCR

Authors

First Name

N. Y.

Last Name

Moustafa

MiddleName

-

Affiliation

Food Control Dept., Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt

Email

-

City

-

Orcid

-

First Name

Azza, M.M

Last Name

Deeb

MiddleName

-

Affiliation

Food Control Dept., Faculty of Veterinary Medicine, Kafrelsheikh Univ., Egypt.

Email

-

City

-

Orcid

-

First Name

Seham, N.

Last Name

Homouda

MiddleName

-

Affiliation

Animal Health Research Institute, Tanta Lab., Tanta, Egypt

Email

-

City

-

Orcid

-

First Name

Shimaa, M.

Last Name

Abd El-Bar

MiddleName

-

Affiliation

Animal Health Research Institute, Tanta Lab., Tanta, Egypt

Email

-

City

-

Orcid

-

Volume

14

Article Issue

1

Related Issue

16421

Issue Date

2016-05-01

Receive Date

2016-02-01

Publish Date

2016-05-01

Page Start

47

Page End

58

Print ISSN

1687-1456

Online ISSN

2682-2954

Article File

KVMJ_Volume 14_Issue 1_Pages 47-58.pdf

PDF . 663.4kB

Link

https://kvmj.journals.ekb.eg/article_108554.html

Detail API

https://kvmj.journals.ekb.eg/service?article_code=108554

Order

3

Type

Original Article

Type Code

1,064

Publication Type

Journal

Publication Title

Kafrelsheikh Veterinary Medical Journal

Publication Link

https://kvmj.journals.ekb.eg/

MainTitle

DETECTION OF STAPHYLOCOCCUS AUREUS CLASSIC ENTEROTOXIN GENES IN SOME MEAT PRODUCTS USING MULTIPLEX PCR

Details

Type

Article

Created At

22 Jan 2023