Egg drop syndrome — 76 (EDS-76) virus was propagated for three passages on embryonated duck eggs followed by thirty passages on C.E.F cell culture, then, for ten passages on SPF embryonated chicken eggs. The titer and HA activity of the 10th passage was 10⁹⁵/ml and 2¹⁰/m1 successively. Inactivation of passage number 10 of propagated virus on SPF-ECE with formaline was adjuvanted with paraffin oil. The prepared vaccine was compared with inactivated oil emulsion EDS76 prepared on embryonated duck eggs for sterility, safety and potency. The potency test was performed by measuring the cellular and humoral immune response. The efficiency of the prepared vaccine was estimated up to six months.