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189417

TRIALS FOR PROPAGATION AND ADAPTATION OF EGG DROP SYNDROME VIRUS EDS-76 ON CHICKEN EMBRYO FIBROBLAST CELL CULTURE AND SPF EMBRYONATED CHICKEN EGGS

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Last updated: 24 Dec 2024

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Abstract

Duck embryo adapted strain of EDS-76 (E11350 106/ml) was propagated and adapted on chicken embryo fibroblast. Thirty serial passages were prepared and titrated. After every 5 passages, the virus was inoculated into chickens 21 days old and observed for 21 days post-infection, then, challenged with a virulent EDS-76 virus. The 24a passage induced complete loss of virus pathogenidty and gave 100% protection, and used for the preparation of a live attenuated and Inactivated oil emulsion chicken embryo fibroblast (CEF) cell culture (EDSV) vacdnes. The evaluation of the prepared vaccines was carried out for sterility, safety and potency. The potency test was performed by measuring the cellular and humeral immune response, as well as, the protection percentage after challenge. The efficiency of the prepared vaccine was estimated up to six months. Passage number 15 and passage number 30 of EDS virus adapted on CEF cell culture were inoculated on SPF embryonated chicken eggs propagation and titration for successive seven passages. Haemagglutination (HA) was estimated to collected materials (allantoic fluids-chorioallantoic membrane-embryonic extract) by rapid haemagglutinating EDS virus and slow quantitative Haemagglutination reaction

DOI

10.21608/ejar.2010.189417

Authors

First Name

NADIA M.

Last Name

IBRAHIM

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Affiliation

Veterinary Serum and Vaccine Research Institute, Agricultural Research Center, Ministry of Agriculture, Dokki, Giza, Egypt.

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Orcid

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First Name

ABO ZAID A.

Last Name

ABO ZAID

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Affiliation

Veterinary Serum and Vaccine Research Institute, Agricultural Research Center, Ministry of Agriculture, Dokki, Giza, Egypt.

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Volume

88

Article Issue

3

Related Issue

27082

Issue Date

2010-09-01

Receive Date

2010-01-13

Publish Date

2010-09-01

Page Start

937

Page End

950

Print ISSN

1110-6336

Online ISSN

2812-4936

Article File

EJAR_Volume 88_Issue 3_Pages 937-950.pdf

PDF . 3.9MB

Link

https://ejar.journals.ekb.eg/article_189417.html

Detail API

https://ejar.journals.ekb.eg/service?article_code=189417

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45

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Original Article

Type Code

1,041

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Journal

Publication Title

Egyptian Journal of Agricultural Research

Publication Link

https://ejar.journals.ekb.eg/

MainTitle

TRIALS FOR PROPAGATION AND ADAPTATION OF EGG DROP SYNDROME VIRUS EDS-76 ON CHICKEN EMBRYO FIBROBLAST CELL CULTURE AND SPF EMBRYONATED CHICKEN EGGS

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Article

Created At

22 Jan 2023