the experimental work was done at Sids experimental farm (Beni Suef Governorate), Animal Production Research Institute, Agricultural Research Center, Egypt. The work was conducted to examine the role of supplemented diets to alleviate effects of waves of cold stress on pregnant buffaloes. Ten buffaloes at late gestation were available for experimental work, their production range between the 4th and 5th parity. Animals were divided into two groups, group A served as control the mean of body weight was 510.0±54.0 Kg and group B with 491.52±43.22 Kg body weight were treated by adding biological additive (ZAD) product to concentrates. Climatic conditions were measured by recording air temperature (AT, °C), relative humidity (RH, %) around animals on weekly basis to calculate the temperature humidity index (THI). Value of THI index was 58.64±0.1 throughout January - Marsh where buffaloes were pregnant while it was 69.33±0.38 at time of delivery during April - June. Adaptive responses including rectal temperature (RT, °C) and respiration rate (RR, (r/min) before blood sampling, and recorded heat dissipation (HD, °C). Blood samples were collected in the morning before feeding. The measurements were taken on biweekly basis in addition to monthly weight of dams (WD, Kg), weight of calves at birth (BW, Kg) and monthly weight of calves and daily gain (DG, Kg). In addition nutrient digestibility and milk composition were estimated. Buffaloes of the treated group B hadthe ability to maintain their heat balance since values of RR and HD were significantly (P ≤ 0.05) less than that of group A particularly after calving. Group B had higher (P ≤ 0.05) concentrations of total protein, albumin and globulin than that of group A after calving and at time of milk peak. In positive response, ZAD treatment increased activity of the transaminases ALT and AST as well as level of total lipids. While urea and creatinine concentrations were increasing in blood of the control group A after calving up to the time of peak, it was decreasing in blood of ZAD treated buffaloes (group B). Concentrations of the thyroid hormones in group B at the time of peak production were relatively higher than the corresponding values before calving or values of group A. significant (P ≤ 0.05) increase in daily milk yield and milk composition of group B was observed in comparison with the control group A. Estimates of feed conversion showed that group B was better than group A.