this work investigated mass production of plantlets from Salvadora persica (Meswak or tooth brush tree). For establishment stage three types of media (MS, WPM or B5), as well as two types of explants (shoot tip or nodal explant) were tested. For multiplication stage of subcultures and different combinations of BAP, KIN. and IBA were studied. Data showed that MS or WPM media gave significantly increased survival percentage comparing with B5 medium. Nodal explant recorded the greatest number of shootlets per explant. While, raising number of subcultures from one to three subcultures led to reducing the survival% from (97.78 to 89.78 and 80.44%) for two and three times of subcultures, respectively. However, the number of shootlets and leaves per explant were increased by increasing number of subcultures. A nonsignificant difference was found in shootlets length due to raising number of subcultures. The greatest results of root formation were occurred in the presence of activated charcoal and IBA. Rooted plantlets were transferred to greenhouse conditions after acclimatization with a success rate of 80%.