illnia indica is a very important medicinal unique tree grown in Egypt (a good source of betulenic acid). An experiment has been conducted to establish a safe protocol for minimal growth conservation using osmosis and growth retardants. Data revealed that abscisic acid (as retardant) (ABA) at 1, 2 or 3 mg/L can be able to save plant life at percentages of 66.67, 83.33 and 50, respectively up to nine months without subculturing. On the other hand, sorbitol (an osmotic substance) has a bad effect especially at high concentrations (6 and 9%) which is lead to 100% death after six months. The results show that the survived shootlets exhibited regeneration ability significantly differed after subculturing into fresh medium. At the end of conservation period (9 months), the regeneration ability of explants derived from shootlets conserved on medium augmented with 1.0 or 2.0 mg/L ABA recorded 100%. When the dendogram was constructed based on the obtained band patterns, results revealed that both the negative (mother plant) and positive controls (in vitro propagated plants) were grouped in separate cluster. Whenever, samples treated with abscisic acid at 1.0 and 3.0 mg/l are grouped in one cluster, which indicated that they are much closed to each other and ABA is genetically safe.