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271844

Bio- and Phyto-chemical Study on Nannochloropsis oculata algal Extract Incorporated with Gold Nanoparticles, in Vitro Study

Article

Last updated: 24 Dec 2024

Subjects

-

Tags

Biochemistry

Abstract

Background: Nannochloropsis oculata is rich in various active phyto-constituents have high ability to diminish generation of free radicals. These components have high molecular weights. Therefore, they integrated with nanotechnology to facilitate their absorption and hence increase their bioavailability. The present experiment was designed to evaluate role of gold nanoparticles (Au-NPs) incorporation in ameliorating the biological efficiency of N. oculata algal extract. Materials and Methods: The ultraviolet-visible (UV-VIS) spectroscopy, X-Ray Diffraction (XRD) spectrum, dynamic light scattering (DLS) measurement and transmission electron microscope (TEM) were used for characterizing the biosynthesized Au-NPs. The major phyto-constituents and the in vitro biological activities (total antioxidant, free radicals scavenging, anti-diabetic and anti-Alzheimer's activities) were quantified in N. oculata algal extract. The cytotoxic activity against growth and progression of human colon carcinoma (CACO-2) and hepatocellular (HEPG-2) cells was assayed. Also, safety of the algal extract after incorporating Au-NPs was evaluated by determining values of the median lethal dose (LD50) and compared with that of the extract itself. Results: It was found that the in vitro biological efficacy (antioxidant, scavenging, anti-diabetic and anti-Alzheimer's activities) increased in N. oculata algal extract after incorporating Au-NPs and this was related to elevating concentrations of the active phyto-constituents. Moreover, it ameliorated the cytotoxic activity against CACO-2 and HEPG-2 cells. Moreover, gold algal nano-extract was orally safer as compared to native algal extract itself. Conclusion: Incorporation of Au-NPs enhanced the in vitro biological activities of N. oculata algal extract.

DOI

10.21608/fsrt.2022.170128.1073

Keywords

Nannochloropsis oculata, green nanotechnology, Gold nanoparticles, Biological Activity, toxicity

Authors

First Name

Wael

Last Name

Aboulthana

MiddleName

Mahmoud

Affiliation

33 El Bohouth St. (Former El Tahrir St.), P.O. 12622, Dokki, Giza, Egypt

Email

wmkamel83@hotmail.com

City

Giza

Orcid

0000-0002-4848-0731

First Name

Randa

Last Name

Bekheit

MiddleName

Abdel-Salam

Affiliation

Chemistry Department (Biochemistry Branch), Faculty of Science, Suez University, P.O. 43518, Suez, Egypt.

Email

randaabdelsalam751997@gmail.com

City

Suez

Orcid

-

First Name

Waleed

Last Name

Serag

MiddleName

Mohamed

Affiliation

Chemistry Department (Biochemistry Branch), Faculty of Science, Suez University, P.O. 43518, Suez, Egypt.

Email

waleedserag3@yahoo.com

City

Suez

Orcid

-

Volume

5

Article Issue

1

Related Issue

37995

Issue Date

2023-03-01

Receive Date

2022-11-12

Publish Date

2023-03-01

Print ISSN

2682-2962

Online ISSN

2682-2970

Link

https://fsrt.journals.ekb.eg/article_271844.html

Detail API

https://fsrt.journals.ekb.eg/service?article_code=271844

Order

271,844

Type

Original Article

Type Code

1,029

Publication Type

Journal

Publication Title

Frontiers in Scientific Research and Technology

Publication Link

https://fsrt.journals.ekb.eg/

MainTitle

Bio- and Phyto-chemical Study on Nannochloropsis oculata algal Extract Incorporated with Gold Nanoparticles, in Vitro Study

Details

Type

Article

Created At

22 Jan 2023