Beta
ManageSearchChatDiscover
255027

Cytotoxicity Effect Assessment of Theophylline Loaded with Collagen Nanoparticles

Article

Last updated: 24 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Veterinary pharmacology

Abstract

The selective drug delivery of asthma treatment is more challenging. Mullet fish contain several types of medically essential substances, such as collagen, which can be an effective and straightforward method of drug delivery. The present study aimed at extracting collagen from mullet fish scales and forming collagen nanoparticles using ethanol (non-solvent for collagen) to provide a desolvation process and loading of theophylline with the nanoprecipitation method collagen nanoparticles in the presence of glutaraldehyde as a crosslinker. The cytotoxicity effect of theophylline loaded with collagen nanoparticles compared to collagen nanoparticles and free theophylline was evaluated using MTT assay on NHSF cell line at the first dose of 3 mM theophylline with double fold serial dilutions. Our research demonstrated the efficacy of theophylline loading in collagen nanoparticles, and we were assured of the result by energy dispersive X-ray mapping (EDX) and optical microscopy. The order of increase in cytotoxicity was collagen nanoparticles < theophylline loaded with collagen nanoparticles < theophylline for all concentrations. These findings imply that collagen nanoparticles are an effective and safe method to use as a drug delivery system.

DOI

10.21608/djvs.2022.140639.1073

Keywords

Collagen extraction, Nanoparticles, Theophylline, Drug delivery, Cytotoxicity

Authors

First Name

Aya

Last Name

Awad

MiddleName

-

Affiliation

Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, AlBeheira, Egypt

Email

ayaahmedawad@yahoo.com

City

Damanhour

Orcid

-

First Name

Manal

Last Name

Shalaby

MiddleName

-

Affiliation

Department of Medical Biotechnology, Institute of Genetic Engineering, City of Scientific Research and Technological Applications, Alexandria, Egypt.

Email

manalshalaby2870@gmail.com

City

Alexandria

Orcid

-

First Name

Gaber

Last Name

Batiha

MiddleName

-

Affiliation

Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, AlBeheira, Egypt

Email

gaberbatiha@gmail.com

City

Damanhour

Orcid

0000-0002-7817-425X

First Name

Rehab

Last Name

Mady

MiddleName

-

Affiliation

Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, AlBeheira, Egypt

Email

rehabmady1976@gmail.com

City

Damanhour

Orcid

-

First Name

Hayder

Last Name

Al-kuraishy

MiddleName

M

Affiliation

College of Medicine, Mustansiriyiah University, Baghdad, Iraq

Email

hayderm36@yahoo.com

City

-

Orcid

-

First Name

Hazem Mohammed

Last Name

Shaheen

MiddleName

-

Affiliation

Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, AlBeheira, Egypt

Email

dr_hazemshaheen3010@yahoo.com

City

Damanhour

Orcid

0000-0001-5931-0466

Volume

8

Article Issue

2

Related Issue

36507

Issue Date

2022-09-01

Receive Date

2022-05-27

Publish Date

2022-09-01

Page Start

5

Page End

10

Print ISSN

2636-3003

Online ISSN

2636-3011

Article File

DJVS_Volume 8_Issue 2_Pages 5-10.pdf

PDF . 863.6kB

Link

https://djvs.journals.ekb.eg/article_255027.html

Detail API

https://djvs.journals.ekb.eg/service?article_code=255027

Order

255,027

Type

Original Article

Type Code

1,001

Publication Type

Journal

Publication Title

Damanhour Journal of Veterinary Sciences

Publication Link

https://djvs.journals.ekb.eg/

MainTitle

Cytotoxicity Effect Assessment of Theophylline Loaded with Collagen Nanoparticles

Details

Type

Article

Created At

22 Jan 2023