Because of the highly effective endocrinal role of vitamin D (Vit D) as an anti-diabetic, there is a great trend to use of Vit D in both prevention and control of type 2 Diabetes mellitus (T2DM). This is the target of our study in which we have used 40 rats allocated into four groups: a control group, given a normal basal diet, and not treated with any drug. Second group; vitamin D supplemented group given vitamin D Vidrop^® (10 IU/kg) by gavage tube daily and given basal diet throughout the experiment. The third group that was a model of T2DM in rats, given a high-fat diet followed by two successive doses of streptozotocin (STZ; 35 mg/kg, i/p), and they were given Vit D Vidrop^® (10 IU/kg) by gavage tube after induction of T2DM till the end of the experiment. The fourth group, which was also a model of T2DM, was given a high-fat diet followed by two successive doses of STZ (35 mg/kg, i/p) but not treated with vitamin D. The result shows that: Vitamin D Vidrop^® (10 IU/kg/60 days) has significantly decreased levels of fasting blood glucose, improved lipid profile and non-esterified fatty acids (NEFA). So, vitamin D acts by supporting β-cell and increases insulin secretion and sensitivity resulting in a significant reduction of both fasting blood glucose and lipoproteins (except HDL), which is a great result.