Pseudomonas aeruginosa (P. aeruginosa) is considered the most predominant pseudomonas species, causing mortality in chickens of all ages. Two-handed samples were collected from broiler chickens of ages ranging from 1 day to 35 days in Sharkia Province that suffered from respiratory distress, diarrhea, and omphalitis in chicks for isolation of P. aeruginosa and detection of its sensitivity to various antimicrobial agents. The prevalence of P. aeruginosa in broiler chickens was 10%. All the isolates showed beta-hemolytic activity on sheep blood agar. The most effective antibiotics were amikacin (100%) and colistin sulfate (95%), followed by norfloxacin (70%) and ciprofloxacin (60%). The antimicrobial resistance pattern of the isolates revealed that all isolated P. aeruginosa were extensively drug-resistant. Subsequently, a farm is difficult to be cleared from P. aeruginosa because of its high resistance to numerous antibiotics so strict hygienic measures should be applied inside poultry farms for controlling P. aeruginosa.