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249303

Prevalence, Virulence and Antimicrobial Resistance of Salmonella Typhimurium in Retail Chicken Meat

Article

Last updated: 24 Dec 2024

Subjects

-

Tags

Bacteriology, Immunology and Mycology

Abstract

Salmonella Typhimuriumwasisolated from food-borne outbreaks basically. The study pointed to reveal the overall prevalence of Salmonella in retail chicken meat, serotyping of Salmonella isolates to detect Salmonella Typhimuriumprevalence in retail chicken meat, antimicrobial susceptibility testing of recovered Salmonella Typhimuriumisolates, and stn, hilA, and pefA as virulence genes and blaTEM, blaCTX-M, blaSHV, blaCMY, blaDHA, qnrA, aadA1, tetA and sul1 as antimicrobial resistance genes. Out of 75 chicken meat samples, Two samples were positively identified as Salmonella with a total prevalence of 2.7 % (2/75). Salmonella isolates were serologically identified as Salmonella enterica serovar Typhimurium. All confirmed Salmonella isolates showed resistance to tetracycline (tetracycline), penicillins (ampicillin), aminoglycoside (gentamycin and streptomycin), sulphonamide (trimethoprim /sulfamethoxazole) and cephalosporins (cefuroxime). In comparison, 50% of the isolates were resistant to cephalosporins (cefotaxime and ceftazidime), nitrofuran (nitrofurantoin), and fluoroquinolones (levofloxacin and ciprofloxacin). Phenotypically, 50% of recovered isolates showed XRD (extensively drug resistance) to eight classes and 50% showed MRD (multidrug resistance). Concerning pefA, stn, and hilA as virulence genes, stn and hilA were detected in all tested Salmonella Typhimuriumby 100% (2/2), while pefA was not detected (0/2). Furthermore, all the following antimicrobial resistance genes were detected by 100 % as blaTEM, blaCTX-M and blaSHV, blaCMY, aadA1, tetA, and sul1. While blaDHA was not detected but qnrA was detected by 50%. In conclusion, raw retail chicken meat is heavily contaminated with XDR Salmonella Typhimurium. Moreover, important virulence factors in the isolates were also present, expanding the scope for public health concerns due to the potential for cross-contamination with other foods and the eating of undercooked poultry.

DOI

10.21608/scvmj.2022.249303

Keywords

Salmonella typhimurium, Serotyping, Antimicrobial susceptibility testing, Virulence genes, antimicrobial resistance genes

Volume

27

Article Issue

1

Related Issue

35626

Issue Date

2022-06-01

Receive Date

2022-06-08

Publish Date

2022-06-30

Page Start

149

Page End

158

Print ISSN

1110-6298

Online ISSN

2682-3284

Link

https://scvmj.journals.ekb.eg/article_249303.html

Detail API

https://scvmj.journals.ekb.eg/service?article_code=249303

Order

13

Type

Original Article

Type Code

992

Publication Type

Journal

Publication Title

Suez Canal Veterinary Medical Journal. SCVMJ

Publication Link

https://scvmj.journals.ekb.eg/

MainTitle

Prevalence, Virulence and Antimicrobial Resistance of Salmonella Typhimurium in Retail Chicken Meat

Details

Type

Article

Created At

22 Jan 2023