Calf diarrhoea is a multifactorial disease entity that can have severe financial animal welfare implications in both dairy and beef herds. The involvement of bacterial pathogens is the main cause of bloody diarrhoea in calves and causes high mortality and morbidity. This study aimed to isolate the bacteria causing diarrhoea and biochemical identification of the isolated bacteria. Faecal samples obtained from diarrheic calves were tested in this study, including buffalo calves and cattle calves aged from seven days to one year. The incidence varied amongst farms, ranging from 0% to 27.9%. The buffalo calves were most affected than cattle calves with diarrhoea. The calves of age from seven days to three months were the most affected calves with diarrhoea. The most isolated bacteria were E.coli  followed by C.pefringens and Salmonella spp. were the last isolated in this study in bacteriological testing through morphological characters and biochemical identification