Two hundred freshly slaughtered of balady chicken samples were collected aseptically from markets in Port-Said city during the period from September 2016 to September 2018. Nine Salmonella isolates (4.5%) were isolated and therefore serologically identified. The isolated Salmonellae were Salmonella Typhimurium 2/9(22.2%) and SalmonellaMuenster 2/9 (22.2%) which was the most frequently identified. Other serotypes as Salmonella Enteritidis 1/9 (11.1%), Salmonella Blegdam 1/9 (11.1%), Salmonella Anatum1/9(11.1%), Salmonella Lamberhurst1/9(11.1%) and Salmonella Ayinde 1/9 (11.1%) were also identified. The antibiogram revealed highly resistant to Erythromycin and Oxytetracycline by a percentage (100%) followed by Nalidixic acid (44.4%) while it was highly sensitive to Chloramphenicol and Ciprofloxacin by a percentage (100%) followed by Gentamycin (88.9%). Polymerase Chain Reaction detected the virulence genes (stn, sop < /em>B, spvC and bcfC) genes were positive in all tested Salmonella serotypes 7/7 (100%) while (avrA) gene was positive in 6/7 (85.7%) of tested Salmonella serotypes.