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102822

Molecular Detection of Some Virulence Genes of E. Coli Isolated from Broiler Chickens and Ducks at Ismailia Governorate

Article

Last updated: 24 Dec 2024

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Subjects

-

Tags

Bacteriology, Immunology and Mycology

Abstract

This study was conducted to investigate the existence of some virulence genes among Escherichia coli strains isolated from broiler chickens and ducks. A total of 252 samples (liver, heart blood and lung) were collected from diseased and freshly dead broiler chickens (192  organ from 64 bird) and ducks (60 organ from 20 bird) from different poultry farms at Ismailia Governorate, Egypt during period from May 2018 to July 2019. All samples were subjected to bacteriological examination for isolation and identification of E. coli. Eighteen E. coli isolates were subjected for detection of some virulence genes (iss, iutA, pap < /em>C, traT, eaeA and vat) by conventional polymerase chain reaction (cPCR). Out of 252 samples, 203 (80.5%) were positive for E. coli. Virulence genes were detected among 18 selected E. coli strains with variable percentage. All 18 E. coli isolates (100%) were iss, iutA and vat genes positive, 16 (88.8%) were traT gene positive, 5 (27.7%) were eaeA gene positive while, 2 (11%) were pap < /em>C gene positive.

DOI

10.21608/scvmj.2020.102822

Keywords

Virulence genes, Escherichia coli, Broiler chickens, Ducks, cPCR

Authors

First Name

Marwa

Last Name

Hassan

MiddleName

-

Affiliation

Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Suez Canal University

Email

-

City

-

Orcid

-

First Name

Mohamed

Last Name

Moursi

MiddleName

-

Affiliation

Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Egypt.

Email

-

City

-

Orcid

-

First Name

Rana

Last Name

Abd El-Fattah

MiddleName

-

Affiliation

Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Egypt.

Email

-

City

-

Orcid

-

First Name

Mohamed

Last Name

Enany

MiddleName

-

Affiliation

Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Suez Canal University.

Email

enanyeg@yahoo.com

City

-

Orcid

-

Volume

25

Article Issue

1

Related Issue

15549

Issue Date

2020-06-01

Receive Date

2020-02-03

Publish Date

2020-06-01

Page Start

1

Page End

19

Print ISSN

1110-6298

Online ISSN

2682-3284

Article File

SCVMJ_Volume 25_Issue 1_Pages 1-19.pdf

PDF . 730.0kB

Link

https://scvmj.journals.ekb.eg/article_102822.html

Detail API

https://scvmj.journals.ekb.eg/service?article_code=102822

Order

1

Type

Original Article

Type Code

992

Publication Type

Journal

Publication Title

Suez Canal Veterinary Medical Journal. SCVMJ

Publication Link

https://scvmj.journals.ekb.eg/

MainTitle

Molecular Detection of Some Virulence Genes of E. Coli Isolated from Broiler Chickens and Ducks at Ismailia Governorate

Details

Type

Article

Created At

22 Jan 2023