Foodstuffs of animal origin may present hazards, due to bacterial contamination. This study was conducted to determine Escherichia coli in some types of animal source foods (raw milk, raw beef meat and raw poultry meat). Bacteriological examination of total 246 raw food samples of animal origin, raw milk, (105); beef meat (31) and poultry meat (110) were collected from different localities in Ismailia City showed that 64/246 (26.01%) of samples were infected with E. coli. Serological identification of (10)  E. colistrains revealed that they were belonged to O₁₁₁ polyvalent 1; O₄₄  polyvalent 2 (2 strains for each); O₇₈ polyvalent 4; O₁₄₆ polyvalent 2; O₂₆ polyvalent 1; O₁₁₉ polyvalent 1; O₈₆ polyvalent 1 and O₁₈ polyvalent 3 (1 strain for each). E. coli strains showed high susceptible rate to Ciprofloxacin (CIP), (100%) and high resistance (100%) to Ampicillin (AMP), Metronidazole (MTZ), Amoxicillin (AML), and Vancomycin (VAS). Four strains of the isolated E. coli  were submitted to molecular studies for detection of the eaeA (protein intimin), iutA (encoding aerobactin) and iss (increased serum survival protein) genes, by using PCR technique.