A total of 12 fungal species belonging to 8 genera were isolated from various collected and examined samples. Fusarium graminearum was grown on suitable medium for crude toxin production, 2ry metabolites were used for treating experimental animal models (not for control). After treatment period blood samples were collected for hormonal assays, Serum testosterone concentration was greatly reduced but follicle stimulating hormone values showed significant increase in treated samples. Testosterone and follicle-stimulating hormone are potential factors required to obtain male fertility and the process of spermatogenesis in the testis