Background: Olive leaves and oil's beneficial effects are not only related to its high content of oleic acid, but also to the antioxidant potential of polyphenolics. Objectives: The present study was aimed to assess the effects of olive leaves and oil treatments on high sucrose induced oxidative damage in rabbits. Methods: Forty male rabbits were allocated into four groups; control group was kept on normal basal ration, sucrose fed group was received 30% sucrose containing ration, sucrose plus olive leaves group was administered olive leaves 5% in ration beside sucrose 30% and sucrose plus olive oil group was received olive oil 5% in basal ration together with sucrose 30% for 60 days. Results: Feeding high sucrose diet significantly increased total lipid, triacylglycerol, cholesterol and LDL-c levels while decreased HDL-c level with hyperglycemia. High sucrose induced hepatic damage assessed by increasing liver marker enzymes. Increased testosterone, copper levels with decreased serum zinc level were also observed. Furthermore, high sucrose diet caused an induction in oxidative stress biomarkers with subsequent depletion in antioxidant enzymes (glutathione-S-transferase and catalase) and reduced glutathione level in liver, heart and kidneys. On the other hand, treatment with olive leaves or oil improved serum lipid profile, liver functions, glucose, zinc and copper levels but decreased testosterone level. Moreover, olive leaves or oil attenuated lipid peroxidation with increase in the antioxidant enzymes. Conclusion: We concluded that olive leaves as well as oil had improved the biochemical alterations induced by feeding high sucrose diet for long time.