Aim: This study was aimed to evaluate the effect of Henna fillers and silver-nanoparticles (Ag-Nps) fillers on surface properties of PMMA acrylic resin. Subjects and methods: One type of conventional heat-cured acrylic resin (Vertex tm Rapid simplified), Henna with a concentration of 1% by weight (Egyptian Local industry), and silver nanoparticles (Ag-Nps) with a concentration of 0.5% by weight (Nano-gate Company Egypt); were selected for this study. The different fillers were added to the polymer powder and was stirred with an electric mixer before mixing with monomer, mixing, packing, and processing was done using the conventional water bath method. After processing, all specimens were finished and polished, then kept in distilled water till tested. All modified and unmodified (control) specimens were used to measure surface hardness and surface roughness. Statistical data analysis was conducted via SPSS program . Results: Incorporation of Henna and Ag-Nps significantly decrease surface hardness of PMMA acrylic resin. While they insignificantly decrease its surface roughness. Conclusion: Surface hardness of PMMA acrylic resin adversely affected by the incorporation of fillers, while surface roughness improved.