Background: Vitiligo is an acquired idiopathic skin disease that appears as skin white depigmented patches due to loss of melanocyte cells that are responsible for melanin pigment production which gives color to the skin, hairs, retina and mucous membranes. Objective: To assess the metabolic changes (namely cholesterol) in blood and tissues of patients suffering from non-segmental vitiligo. Patients and Methods: Our study was carried out during the period from September 2020 to March 2021. Patients were selected from the outpatient skin clinic at Al-HusseinHospital, Al-AzharUniversity, Cairo. A total number of 30 patients suffering from non-segmental vitiligo were included in addition to 30 normal persons of the same age group and both sexes, not having any skin disease, not suffering from any systemic morbid disease (diabetes mellitus, dyslipidemia, liver disease, kidney disease or hypertension) or taking any systemic medication for any other disease were introduced in the study as a control group.      A Punch skin biopsy was taken from the vitiliginous patches and another one from the adjacent apparently normal skin of the patients. The specimens were stained with special stains for cholesterol (Sudan III) stain. Results: The results of this study disclosed elevated levels of cholesterol in the blood samples of about 21 patients (70%) among the total number of patients, compared to 9 patients (30%) who showed normal blood cholesterol values.      Tissue biopsy specimens from the vitiliginous patches of the patients, stained with Sudan III stain, disclosed the presence of cholesterol in the dermis in 22 patients (73.3 %), while the rest of the specimens of 8 patients (26.7 %) showed no cholesterol deposition.      Tissue biopsy specimens from the apparently normal skin of the patients did not show any lipids in the dermis of these samples. Conclusion: Increased blood cholesterol levels in addition to the presence of cholesterol (as a secondary metabolic product) deposited in the skin tissue samples of vitiliginous patches could be related to nerve fibers which were undergoing a degenerative process. These nerve fibers were innervating the melanocyte cells in the skin.