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277854

Polymorphisms in the exon-3 of leptin gene using tetra-primer ARMS-PCR in Egyptian buffalo

Article

Last updated: 24 Dec 2024

Subjects

-

Tags

Animal Production

Abstract

The Egyptian buffalo is one of the Egyptian genetic resources which is irreplaceable and must be preserved, because of its great adaptability to local harsh environmental conditions and resistance to infectious diseases. A total number of 74 blood samples of female Egyptian buffalo were used to assess the allelic and genotypic frequency of G>A SNP at codon 159 of leptin gene. The tetra-primer amplification refractory mutation system-polymerase chain reaction (Tetra-primer ARMS-PCR), a single nucleotide polymorphism (SNP) technique allows for the simultaneous detection of alleles using a single PCR reaction and gel electrophoresis. Results revealed three genotypes GG, AG, and AA with frequencies of 0.49, 0.39, and 0.12, respectively. Allele frequencies of G and A were found to be 0.69 and 0.31, respectively. For the first time, allelic and genotypic frequency of G>A SNP at codon 159 in Egyptian buffalo was detected with the highest allelic frequency of allele A (0.31). Further studies are needed to provide more information about the association between this SNP and production and reproduction traits.

DOI

10.21608/ajar.2022.277854

Keywords

Egyptian buffalo, Tetra-primer ARMS-PCR, leptin hormone

Authors

First Name

F. A.

Last Name

Sharara

MiddleName

-

Affiliation

Animal Production Department, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt

Email

fahmysharara@azhar.edu.eg

City

-

Orcid

-

Volume

47

Article Issue

2

Related Issue

38609

Issue Date

2022-12-01

Receive Date

2022-08-01

Publish Date

2022-12-01

Page Start

182

Page End

185

Print ISSN

1110-1563

Online ISSN

2786-0051

Link

https://ajar.journals.ekb.eg/article_277854.html

Detail API

https://ajar.journals.ekb.eg/service?article_code=277854

Order

16

Type

Original Article

Type Code

929

Publication Type

Journal

Publication Title

Al-Azhar Journal of Agricultural Research

Publication Link

https://ajar.journals.ekb.eg/

MainTitle

Polymorphisms in the exon-3 of leptin gene using tetra-primer ARMS-PCR in Egyptian buffalo

Details

Type

Article

Created At

22 Jan 2023