Eight isolates of the Cucumber mosaic virus (CMV) were isolated from cucumber (Cucumis sativus) and zucchini (Cucurbita pepo) plants showing symptoms matched the CMV infection. The identity of these isolates was confirmed as CMV based on diagnostic hosts reaction, enzyme-linked immunosorbent assay (ELISA), and electron microscopy. The identification was confirmed at the molecular level. The obtained isolates were mechanically transmitted to diagnostic hosts which produced typical symptoms of CMV infection on each plant. Also, its easily transmitted by aphid (Aphis gossypii) in non-persistent manner. The virus could be transmitted through seeds of pumpkin, zucchini, and cucumber but not through seeds of melon. A variation in aggressiveness between obtained isolates were observed. Evaluating the disease reaction of some cultivated cucurbit types toward CMV artificially infection reveled that all the tested types were susceptible but differed in their susceptibility rate.