Sugar beet (Beta vulgaris L.) ranks second important sugar crop in the world. The aim of the herein trial is to study the effect of mineral and nano-nitrogen fertilizer (Sissay) and a mix of three micro elements (boron, zinc and manganese) on growth and yield in sugar beet plants. Two greenhouse experiments were carried out at the experimental farm of the Faculty of Agriculture, Nasr city, Al-Azhar University, Cairo, Egypt during 2016/2017 and 2017/2018 seasons. The experiments were laid out in a complete randomized block design with four replications. The obtained results showed that the treatment had mineral nitrogen at a rate of 54 or 90 Kg N per feddan + nano Sissay (810 g/ feddan) + a mix of nano micro elements (200 ppm) gave the highest values of relative growth rate, net assimilation rate over 60 to 90 days from sowing date, root weight, sucrose percentage and sugar yield per plant at harvest time as compared with all other studied treatments in both seasons. Control treatment (without fertilization) gave the lowest values. In conclusion, using nano-nitrogen fertilizer (Sissay) and micronutrients (B, Zn and Mn) with mineral nitrogen fertilizer can save around 40% from recommended doses of mineral nitrogen fertilizer.