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102619

PCR assay a new approach for detection of enterotoxins

Article

Last updated: 24 Dec 2024

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Abstract

B. cereus group is considered as a potential problem, since it can contaminate many dairy products. In this study, three primer sets were selected to simultaneously detect two different species of the B. cereus group by using triple – primer PCR. The triple–primer PCR in this study were synthesized using the CER, CES and groEL genes for the detection of emetic toxin producing strains and another specific primer for the detection of diarrheal toxin (groEL gene only). Results indicated that all the diarrheal enterotoxin producing B. cereus strains showed a presence of groEL gene, while CER and CES genes were completely absent. Out of the six B. cereus strains tested for the production of diarrheal and emetic enterotoxins by using triple–primer PCR technique, three diarrheal enterotoxin producing strains were only detected. On the other hand, all six B. cereus strains had limited ability to produce emetic toxin. All diarrheal enterotoxin producing B. mycoides strains showed presence of groEL gene, but CER and CES genes were not detected in any of the B. mycoides tested strains. Consequently, four B. mycoides strains were tested for the production of diarrheal and emetic enterotoxins by using triple–primer PCR technique, only two strains showed diarrheal enterotoxin producing strains. In contrary all B. mycoides tested strains had limited ability to produce emetic toxin.

DOI

10.21608/ajar.2019.102619

Keywords

aerobic spore forming bacteria, milk products, B. cereus group, Enterotoxin producing

Authors

First Name

Khater

Last Name

Khater

MiddleName

Abdelfatah

Affiliation

Dairy Department, Faculty of Agriculture, Al Azhar University, Cairo, Egypt

Email

khaterkhater.5@azhar.edu.eg

City

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Orcid

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First Name

S.

Last Name

Abdella

MiddleName

A.

Affiliation

Dairy Department, Faculty of Agriculture, Al Azhar University, Cairo, Egypt

Email

-

City

-

Orcid

-

Volume

44

Article Issue

2

Related Issue

15338

Issue Date

2019-12-01

Receive Date

2019-07-04

Publish Date

2019-12-01

Page Start

59

Page End

65

Print ISSN

1110-1563

Online ISSN

2786-0051

Link

https://ajar.journals.ekb.eg/article_102619.html

Detail API

https://ajar.journals.ekb.eg/service?article_code=102619

Order

6

Type

Original Article

Type Code

929

Publication Type

Journal

Publication Title

Al-Azhar Journal of Agricultural Research

Publication Link

https://ajar.journals.ekb.eg/

MainTitle

PCR assay a new approach for detection of enterotoxins

Details

Type

Article

Created At

22 Jan 2023