The aim of the present study is to evaluate the effect of modified milk production from goats administrated different amounts of flaxseed oil on serum lipid profile of rats. Twenty-five mature Albino rats were randomly and equally divided into five groups, five rats each. The rats were acclimatized on basal diet for 7 days before starting the experiment. The first group was fed on basal diet and served as control I, the second group was offered basal diet plus normal goat milk (control II), while the rest three groups were fed on basal diet plus milk from goats received 10, 20 and 30 mL flaxseed oil/head/day. Results of serum analysis revealed that serum triglycerides concentrations reduced by 1.42, 6.57 and 14.10% in tested groups G1, G2 and G3 respectively, compared to control II. Moreover, total cholesterol levels were linearly decreased after 28 days of feeding, with reduction values varied from 1.39 to 9.10% with a mean value of 5.48% for treated group, compared to control II. Continuously, LDL-cholesterol levels were decreased by 13.81 and 16.49 in G2 and G3 group respectively, as compared with control II. While no significant was detected for G1 group. However, the same trend of result was also true for VLDL-cholesterol levels. In contrary, at the end of experiment, the levels of HDL–cholesterol as "anti-atherogenic" index were increased in all treatments compared to their corresponding initial values. In conclusion, flaxseed oil can be used as nutritional supplement to lactating goats to reduce saturated fatty acids and increase polyunsaturated fatty acids in the resultant milk.