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257533

Effect of genetic diversity, Antimicrobial activity, and Safety Evaluation Between probiotic isolates From Groups of Enterococcus faecium Isolates

Article

Last updated: 22 Jan 2023

Subjects

-

Tags

Agricultural Microbiology

Abstract

Marine probiotic sh6 and sh7 have been isolated from the Mediterranean and the Red Sea beaches of Egypt, respectivily. Were identified as "Enterococcus faecium AUMC B-450 and AUMC B-451  "by molecular identification These novel strains were compared to (LAB) as a reference for probiotic properties. The two strains were analysed for hemolytic activity the results were non hemolytic blood. The bacteria were discovered to be resistant to the gastrointestinal tract's severe environment (acidity pH3 and bile salt 0.5%). Susceptibility of E. faecium AUMC B-450 and AUMC B-451  strains to some different commercially available antibiotic discs: (tetracycline, penicillin, streptomycin,erythromycin, Nalidixic acid, Ciprofloxacin, chloramphenicol, and gentamicin) have been tested the method of disc diffusion using antibiotics discs. The result demonstrated that E. faecium AUMC B-450 is sensitive to):penicillin-streptomycin-erythromycin-Nalidixic acid-Ciprofloxacin-and chloramphenicol( but Insensitive  to tetracycline  -penicillin  –streptomycin - erythromycin- Nalidixic.acid-Ciprofloxacin-gentamicin -and chloramphenicol ( but E. faecium AUMC B-451 sensitive to)tetracycline-penicillin- streptomycin -erythromycin-Nalidixic acid-Ciprofloxacin and chloramphenicol(but Insensitive to (Nalidixic acid, Ciprofloxacin, chloramphenicol). These two strains E. faecium AUMC B-450 and AUMC B-451  exhibited wide antimicrobial efficacy against the most common Gram-positive , Gram-negative and fungi pathogenic. The degree of its safety the strains was measured by a toxicity test, and it was found that the two strains E. faecium AUMC B-450 and AUMC B-451  have a high degree of safety. According to the findings, E. faecium AUMC B-450 and AUMC B-451 appears to be suitable for application as a probiotic in the food and feed industries in the future.

DOI

10.21608/sjas.2022.148578.1244

Keywords

Enterococcus faecium, probiotics, pathogen inhibition, Cytotoxicity

Authors

First Name

shimaa

Last Name

Ramadan

MiddleName

Ramadan

Affiliation

Microbiology Department ,faculty of Agriculture ,Bani-suif

Email

shimaaramdan36@gmail.com

City

Beni-suef

Orcid

0000-0001-8101-6757

First Name

shimaa

Last Name

ali

MiddleName

k.

Affiliation

microbiology Department ,faculty of agriculture , bani-suif

Email

shimaahmdeen66@gmail.com

City

-

Orcid

-

First Name

Amany

Last Name

Basuny

MiddleName

M

Affiliation

Biochemistry Department ,Faculty of Agriculture ,Beni-Suef University

Email

amanymohamedgg@gmail.com

City

Beni-suef

Orcid

-

First Name

Alshaymaa

Last Name

ahmed

MiddleName

I.

Affiliation

Microbiology Department ,faculty of agriculture ,bani-suif

Email

shimaamohamed@gmail.com

City

Beni-suef

Orcid

-

Volume

4

Article Issue

2

Related Issue

36486

Issue Date

2022-09-01

Receive Date

2022-07-02

Publish Date

2022-09-01

Page Start

1

Page End

10

Print ISSN

2535-1796

Online ISSN

2535-180X

Link

https://sjas.journals.ekb.eg/article_257533.html

Detail API

https://sjas.journals.ekb.eg/service?article_code=257533

Order

1

Type

Original Article

Type Code

916

Publication Type

Journal

Publication Title

Scientific Journal of Agricultural Sciences

Publication Link

https://sjas.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023