The aim of the study was directed to investigate the bacteriological quality of raw milk in Beni-Suef governorate, Egypt by conventional bacteriological methods. A total of 125 raw milk samples including 25 bulk tank milk of large dairy herds, 25 bulk tank milk of large dairy herd (after improving the environmental and pre milking sanitation), 25 milk samples from smallholder dairy herds, and 50 samples from milk shops, were collected from Beni-Suef governorate, Egypt. All samples were examined for Total Bacterial Count (TBC), Psychrotrophic Count (PC), Total Coliform Count (TCC), Fecal coliform (FC), and E.coli. The mean values of TBC were 1.53 ×107 ± 6.27×106, 2×106 ± 6.58×105, 7.85 × 106 ± 3.95 × 106, 1.65 ×107 ± 4.32 ×106 cfu/ml, respectively. The mean values of PC were 2.83 × 105 ± 6.85 × 104, 8.98 × 104 ± 2.67 × 104, 6.85 × 104 ± 1.24 × 104, and 1.72 x 105 ± 3.79 × 104 cfu/ml, respectively. The mean values of TCC were 3.89 ×105± 1.38 ×105, 5.3 × 104 ± 3.07×104,  6.4 × 105± 1.57 ×105, 2.2 × 106 ± 6.87 ×105 MPN/ ml, respectively. The mean values of FC were  9.20 × 103 ± 1.82 × 103, 6.36 × 102 ± 1.9 × 102, 6.04 × 103 ± 1.57×103, 6.5×103 ± 1.08 ×103 MPN/ ml, respectively. The mean values of E.coli were 2.57 × 103± 6.11 × 102, 3.8 × 102 ± 2.48 × 102, 3.89 × 103 ± 7.36 × 102, 4.15 × 103 ± 5.44 × 102 cfu/ml, respectively. The results showed that improving sanitation significantly improved milk quality. High bacterial counts in raw milk especially E.coli constitute a public health hazard to the consumers and emphasize the great need for an improved hygienic standard.