Laboratory, greenhouse and field experiments were preformed to evaluate the antifungal activity of cobalt sulphate, salicylic acid, ethephone, dipotassium phosphate, ascorbic acid and neem oil at three concentrations for each against Sclerotinia sclerotiorum growth and sclerotial formation on PDA (Potato Dextrose Agar) and to verify its positive efficacy as defence activators (elicitors) in chickpea seedling and plants against damping-off and white stem rot disease. Also, efficacy of the five elicitors and neem oil in improving some growth parameters, protein, nitrogen, potassium and phosphorus contents of seed was determined.
1-Neem oil (2.5, 5.0 or 7.5 ml L-1) and salicylic acid at higher concentration (7.5 mM) significantly reduced the fungal growth and number of sclerotial formation in vitro. Reduction was always increased by increasing concentration of neem oil.
2-Soaking seeds in each one of the five elicitors or the neem oil tested, except ethephone, decreased seedling damping-off pre- (in most cases) or post-emergence and increased survivals. The most effective inducers, however, were cobalt sulphate (1 mg L-1), salicylic acid (7.5 mM), neem oil (5 ml L-1), dipotassium phosphate (5 mM) and ascorbic acid (10 mM). While, ethephone treatment was the least effective. In the field experiments, white stem rot disease incidence was significantly minimized with all tessted treatments tested in both seasons, except ethephone. The highest reduction in the disease incidence was recorded with neem oil, followed by dipotassium phosphate and cobalt sulphate.
3- Giza 2 cv. was the highest susceptible to infection under greenhouse and field conditions. While, Giza195 and Giza 531 cvs. were the least.
4- Results of the field experiments indicated that application of all treatments, except ethephone, resulted in a significant improve in crop parameters of chickpea cultivars compared with the untreated control. Dipotassium phosphate recorded the highest number of tillers, weight of seed yield per plant, number of capsules per plant, and seed yield / feddan.
5- Giza 1 cultivar had the higher value of seed yield/fed. In the 1st season, whereas Giza195 cultivar in the 2nd season.
6- Giza 195 seeds had the higher crude protein content in the 1st season, while Giza 531 showed the higher protein content in the 2nd season.
7- Giza1 seeds had the higher content in phosphorus and potassium in the two seasons.
8- Ethephone exhibited the highest reduction in plant height, 100-seed weight, seed yield per feddan and N,P and K content in seeds..
9- Dipotassium phosphate and neem oil treatments were superior than the other in increasing values of seed yield/fed., nitrogen, protein, phosphorus, potassium contents in Giza1, and Giza 195 cvs., while the lowest of them was detected in Giza2.