This investigation was conducted at the apiary of Sakha Agricultural Research Station to evaluate certain factors affecting protein content in the ovaries of virgin queens and nitrogen content in different body regions (head, thoray and abdomen) of virgin queens. Major factors affecting protein in queen ovaries were type of cups used in rearing the larvae , with plastic cups are better than wax ones . the highest nitrogen value was recorded in soybean diet with  plastic cups , with a mean value of  3.372 %, while the lowest value was recorded in natural pollen grains in wax cups with a queen head value of 0.521 %. The highest nitrogen value was recorded in soybean diet offered in plastic cups with a queen thorax value of 3.146., the lowest value was recorded in natural pollen grains offered in wax queen head with mean value of 0.446. The effects of larval ages and different seasons during queen rearing were studied.