The present study will give us deeper insights on the Syrian truffles which are one of the miracle of nature. Some components of Syrian truffle (Tuber brumale vittadini)  were estimated in terms ofnutritional value. The effects of Syrian truffle (ST)  on the normal and onset of alloxan-induced diabetes in male Swiss albino mice were examined. It was observed that the mouse given orally extract of  ST (10 ppm for 12 weeks) showed a significant decrease in the blood glucose level and glycosylatedhemoglobin A1c of diabetic mice induced by intra-peritoneal injection of alloxan (50 mg/ kg, i.p.) and significantly improved the glucose tolerance test.  Tuber brumale vittadini may normalize blood AST, ALT, creatinine and cholesterol in alloxan induced-diabetic group. Histolochemical examinations were also done on liver and kidney.  Oral administration of 10 ppm ST extract,3 times weekly  for 12 weeks of alloxan diabetic mice showed decrease in collagen fibers in liver and kidney tissues and  increase in liver glycogen content whereas decreased glycogen in the kidney as compared with the control group. These findings revealed that extract of ST may have a potential benefit in the treatment of diabetes, play a role in its modulatinghepatic and renal structural changes in diabetic Swiss albino mice  and may reduces the risk of diabetic complications.