Sporulation of Alternaria solani (Ellis and Martin) Jones and Grout, can be scarce and is often reduced when the fungus is cultivated in-vitro. An experiment was conducted to assess the effect of culture media on mycelial growth and sporulation of two isolates of A. solani namely Badr and Al-Tawfiqiyah, in-vitro. Badr isolate gave the highest mycelial linear growth when grown on potato dextrose agar (PDA) and V-8 media. However, it produced maximum yield of conidia on V-8 medium. Al-Tawfiqiyah isolate was best grown but least sporulated on S-medium and PDA. However, its maximum yield of spores was obtained on V-8 medium. Thus, V-8 medium was the best among all media tested for sporulation of both A. solani isolates.